The sensor chip surface must be selected correctly according to various conditions such as analyte type, analyte size, ligand immobilization method, and non-specific adsorption.
Alkanthiols based planar sensor chips
Since the iMSPR system has sufficient sensitivity, satisfactory results can be obtained even on a sensor chip (Planar Au sensor chip) formed with a monolayer of linker material that can immobilize the ligand in the case of a molecule with a relatively large size (about 5 kDa or more) – protein, cell, bacteria, virus, exosome, etc. In particular, in the case of antibody drugs, it is a very suitable application field because the signal is large and the affinity is high.
Structure
Immobilization level (Max.)
100 ~ 2000 RU (molecule type and MW dependent)
Dextran based membrane sensor chips
Dextran sensor chips were first commercially available in the early 1990s by applying solid surface technology well-optimized for affinity chromatography. Therefore, the largest amount of data has been accumulated using SPR to date. Therefore, there are many literatures and results that can be referenced, which is very advantageous for reproducing similar experiments. It is a universal sensor chip that can be used from large-sized proteins to small compounds.
Structure
Immobilization level (Max.)
1000 ~ 10000 RU (molecule type and MW dependent)
Linear polymer based membrane sensor chips
The linear polymer sensor chip developed this time is a sensor chip that complements the shortcomings of the dextran sensor chip while maintaining the performance of the membrane sensor chip. It can be fabricated more reproducibly, exhibits a higher signal-to-noise ratio, improves diffusion properties and reduces non-specific adsorption. A representative sensor chip is the HC1000. It is a sensor chip suitable for analyzing small molecular compounds.
Structure
Immobilization level (Max.)
1000 ~ 20000 RU (molecule type and MW dependent)
Contact angle comparison
Sensor chips have different degrees of hydrophilicity depending on the backborn material structure. Instead of using just one chip, improve the quality of your analysis with sensor chips of various surfaces.
Sensor chip storage kit
It is an essential tool to keep your sensor chip running. After removing the used prism holder from the device, install the s-type fluidics module. Then fill with storage buffer and store in the refrigerator. The ligand material immobilized on the sensor chip can be safely stored until the next use.
