CELENA® X High Content Imaging System

CX30004

Automated high content image acquisition and analysis for drug discovery and cell biology

The CELENA® X High Content Imaging System is an advanced, integrated platform designed to streamline high content image acquisition and analysis with remarkable speed and precision. Its customizable imaging protocols, combined with image-based and laser autofocusing modules, facilitate efficient well plate imaging and slide scanning. The motorized XYZ stage enhances automation, while interchangeable filter cubes and objectives offer flexibility for a wide range of fixed and live cell imaging applications. The system’s integrated CELENA® X Cell Analyzer software processes both images and data, enabling comprehensive quantitative analysis. Users can create and save analysis pipelines to identify cellular or subcellular objects, optimize image processing, and perform various measurements with ease.

The CELENA® X excels in fully automated plate and slide imaging, featuring automated vessel handling and scanning through a motorized XYZ stage, filter cube stage, and objective turret. Its laser autofocus ensures rapid, reproducible focusing while minimizing phototoxicity and photobleaching, which is crucial for maintaining cell viability during imaging. For live cell assays, the onstage incubation system provides precise control over physiological and non-physiological conditions, supporting a variety of experimental setups. The system supports four imaging modes: fluorescence in four channels, brightfield, color brightfield, and phase contrast, accommodating diverse imaging needs and experimental requirements.

The CELENA® X’s powerful and user-friendly software interface simplifies the setup of imaging protocols and integrates seamlessly with data analysis processes. Its customizable high content analysis capabilities allow for the creation of tailored workflows that can handle both straightforward fixed cell assays and complex, time-lapse live cell assays. The system supports a wide array of applications, including cell counting, cytotoxicity assays, transfection efficiency, wound healing, apoptosis, confluency, calcium signaling, phagocytosis, cell cycle analysis, stitching, and 3D cell modeling. The CELENA® X is designed to be versatile and adaptable, with comprehensive vessel holder options, objective selection guides, and LED filter cube guides available to ensure that the system meets all of your cell imaging needs.

Specifications

Supported labware Slides, multi-well plates (6 to 1536 wells), petri dishes, culture flasks
Imaging modes 4-channel fluorescence, brightfield, phase contrast, color brightfield
Light source High-power LED filter cubes with adjustable intensity (>50,000 hours per filter cube)
Filter cube stage Motorized; 4 interchangeable fluorescence filter cubes and 1 brightfield filter cube
Available filters DAPI, EGFP, RFP, mCherry, ECFP, EYFP, DSRed, Cy5, Cy7, Cy3/TRITC Long Pass, GFP Long Pass, Cy5 Long Pass, custom filters
Objective turret Motorized; 5 interchangeable objectives
Compatible objectives 1.25-100X; Olympus, Zeiss, and Logos Biosystems objectives
Condenser Motorized; basic or phase contrast
Basic: 60 mm LWD condenser, 4 positions
Phase contrast: 60 mm LWD condenser, 4 positions with 3 phase annuli
Camera Monochrome: CMOS, 1.92 MP
(optional) Color: CMOS, 1.92 MP
Image outputs Monochrome: 16‐bit (12‐bit dynamic range) TIF, PNG, or JPG
Color: 24-bit color TIF, PNG, or JPG
Movies: MP4
Autofocus method Image-based autofocus
(optional) Laser autofocus
Stage Motorized X/Y-stage (120 mm x 80 mm); motorized Z-stage (10 mm)
Stage control CELENA® X Explorer
(optional) Joystick
Computer External PC
Monitor 27” 4K UHD monitor
Software User interface: CELENA® X Explorer
Analysis: CELENA® X Cell Analyzer
Power 100-240 VAC, 250 W, 50/60 Hz
Dimensions Main body: 39 x 46 x 50 cm (15.4 x 18.1 x 19.7 in)
Controller: 17 x 30 x 23 cm (6.7 x 11.8 x 9.1 in)
Weight Main body: 33 kg (72.8 lbs)
Controller: 7 kg (15.4 lbs)

Resources

Ordering Information

Cat # Product Qty
CX30000 CELENA® X High Content Imaging System 1 unit
I10530 CX Stage Top Incubator, Basic [Tokai] 1 set
I10531 CX Stage Top Incubator, Pro [Tokai] 1 set
I10500 Heating System for Multi-well Plates [Ibidi] 1 set
I10502 Gas Incubation System for CO2 [Ibidi] 1 set
I10503 Gas Incubation System for CO2/O2 [Ibidi] 1 set
I10030 UPLFLN 4X, Olympus 1 unit
I10031 UPLFLN 10X2, Olympus 1 unit
I10034 LUCPLFLN 20X, Olympus 1 unit
I10035 LUCPLFLN 40X, Olympus 1 unit
I10038 UPLFLN 4XPH, Olympus 1 unit
I10039 UPLFLN 10X2PH, Olympus 1 unit
I10042 LUCPLFLN 20XPH, Olympus 1 unit
I10043 LUCPLFLN 40XPH, Olympus 1 unit
I10046 PLAPON 1.25X, Olympus 1 unit
I10047 PLAPON 2X, Olympus 1 unit
I10052 UPLXAPO 60XO, Olympus 1 unit
I10051 UPLXAPO 100XO, Olympus 1 unit
I10130 DAPI (Ex395/25, Em460/50) 1 unit
I10131 EGFP (Ex470/30, Em530/50) 1 unit
I10132 RFP (Ex530/40, Em605/55) 1 unit
I10133 mCherry (Ex580/25, Em645/75) 1 unit
I10134 ECFP (Ex436/20, Em480/40) 1 unit
I10135 EYFP (Ex500/20, Em535/30) 1 unit
I10136 DSRed (Ex530/40, Em620/60) 1 unit
I10137 Cy5 (Ex620/60, Em700/75) 1 unit
I10138 Cy7 (Ex710/75, Em810/90) 1 unit
I10139 Cy3/TRITC Long Pass (Ex530/40, Em570lp) 1 unit
I10140 GFP Long Pass (Ex470/40, Em500lp) 1 unit
I10141 Cy5 Long Pass (Ex620/60, Em665lp) 1 unit
I10142 Custom Filters 1 unit
I10410 Joystick 1 unit

 

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