Accurate cell concentration and viability are essential for high-quality downstream procedures, especially with tissue-derived cells like stem cells or those from the adipose stromal vascular fraction (SVF). Contamination with red blood cells and debris can complicate counting. The LUNA-STEM™ overcomes these challenges with dual fluorescence and brightfield optics, effectively differentiating live and dead nucleated cells from non-nucleated cells. Staining with nucleic acid dyes allows precise measurement of cell concentration and viability. Within 30 seconds, it provides total and nucleated cell concentrations, viability, and average cell size. High-resolution images visually confirm data, with detailed PDF reports available for transfer or printing.