Designed to Empower Deep Scientific Insights
The ID7000™ spectral cell analyzer represents a significant advancement in high-parameter flow cytometry, offering unparalleled capabilities for comprehensive cell analysis. This state-of-the-art system excels in detecting both dim and rare cell populations with high sensitivity, making it a valuable tool for researchers aiming to explore complex biological questions. Equipped with up to seven lasers and 186 detectors—the most of any flow cytometer currently available—the ID7000 supports experiments with 44 colors or more, depending on the fluorochromes used. This extensive configuration allows for the detailed examination of heterogeneous cell populations and accommodates future advancements in fluorochrome technology. The laser options range from deep ultraviolet (320 nm) to infrared (808 nm), ensuring versatility and the capability to expand multicolor panels. The system’s advanced detection features facilitate precise and accurate data collection, streamlining high-parameter experiments and multicolor workflows. Innovative elements such as the Spectral Reference Library and the Autofluorescence Finder enhance data fidelity by identifying and subtracting autofluorescence contributions, thus ensuring more accurate and reliable results.
Designed for Multicolor Workflows
The ID7000’s advanced optics design, alongside proprietary technologies, delivers exceptional data quality and resolution. Sony has leveraged its expertise in spectral technology to develop signal processing methods that minimize electronic noise, which is crucial for achieving high sensitivity in flow cytometry. This high sensitivity enables the accurate detection of rare and dim signals, thus facilitating deeper insights into cellular biology. The new excitation and detection optics are tailored to resolve spectrally similar fluorochromes with precision. The system features three standard excitation lasers—488 nm (blue), 637 nm (red), and 405 nm (violet)—with optional lasers at 561 nm (yellow-green), 355 nm (ultraviolet), 320 nm (deep ultraviolet), and 808 nm (infrared). Each laser is optimized to maximize performance for detecting various markers, including those with low signal intensity.
The expanded availability of detectors is designed to handle the complexity of panel design and fluorochrome selection, empowering researchers to push the boundaries of discovery. The ID7000 employs photomultiplier tubes (PMTs) known for their high signal-to-noise ratios to capture photons effectively. Emitted light is collected across a wide spectrum (360 nm to 920 nm) using an innovative light diffraction grating-based collection system. This system enhances photon capture and provides high resolution, ensuring that even the faintest signals are detected. By distributing light evenly across the detector array, the system minimizes light loss and maximizes data accuracy.
Designed to Harness the Power of Spectral Data
The ID7000 spectral cell analyzer simplifies complex operations and enhances the accuracy of spectral analysis. Spectral unmixing, a key feature of the ID7000, separates overlapping signals into pure fluorochrome signals, enabling precise measurement of each marker. The system uses the Weighted Least Squares Method (WLSM) to perform unmixing, which is essential for analyzing dim and rare markers. This technology simplifies the design of multicolor panels by eliminating the need for traditional bandpass filters and compensation matrixes, reducing complexity and potential errors in data interpretation.
Additionally, the ID7000 addresses the challenge of autofluorescence, which can interfere with the signals from fluorescent markers. Spectral technology enables autofluorescence to be treated as a separate color, allowing for accurate subtraction and clearer visualization of true fluorescent populations. The system’s Spectral Reference Library stores single-color control data, which is used to enhance the unmixing process. This library eliminates the need for repetitive controls in each experiment, saving time and resources.
The ID7000 also features a standardization mode to ensure reproducible data across experiments and instruments. This mode optimizes instrument settings to maintain consistency, supporting longitudinal studies and cross-site collaborations. By adjusting PMT voltages and other parameters, the system minimizes variability and enhances data reliability.
Designed for Automated Sample Acquisition
Automation is a core feature of the ID7000, designed to streamline operations and ensure accurate results. The advanced AutoSampler accommodates various plate and tube formats, including 5-mL tube racks and 96-well plates, and allows for sample cooling and agitation. The AutoSampler’s flexibility includes options for different wash protocols and sample handling modes, ensuring that samples remain stable and accurate throughout the acquisition process. Additional features include an extra loading station for QC particles or cleaning solutions, enhancing convenience and efficiency during multi-sample acquisitions.
The ID7000’s software is user-friendly, guiding researchers through setup, acquisition, and analysis with ease. It offers expert and guided modes to accommodate users with varying levels of experience. The software supports customizable workflows and automated data acquisition, reducing the need for manual intervention and allowing researchers to focus on their scientific work.
Automated Setup and Quality Control
The ID7000 includes automated setup and quality control features to ensure optimal performance. At startup, the system uses wizards for alignments and performance checks, providing real-time feedback and generating reports for tracking instrument performance. The History and Trend views in the QC tab allow users to monitor performance over time, ensuring consistency and reliability in data collection. These features are crucial for maintaining high standards in research and supporting reproducible results across different experiments and laboratories.
Overall, the ID7000 spectral cell analyzer combines cutting-edge spectral technology with robust automation and user-friendly software, making it an ideal choice for researchers seeking to advance their work in high-parameter flow cytometry. Its ability to handle complex multicolor workflows, combined with powerful data analysis tools and automation, positions it as a leading solution for exploring and understanding cellular biology in unprecedented detail.
