The Sony SA3800 spectral cell analyzer represents a groundbreaking advancement in flow cytometry, integrating sophisticated electronics and patented optical technologies to deliver exceptional analytical performance. Designed to streamline the entire workflow, from instrument startup to system maintenance, the SA3800 offers unparalleled automation and simplicity, making it suitable for both seasoned experts and newcomers to flow cytometry.

Advanced Spectral Technology

At the core of the SA3800’s capabilities is its spectral analysis technology, which significantly enhances sensitivity and facilitates the detection of dim signals. By capturing photons across a broad wavelength range from 420 nm to 800 nm, the SA3800 optimizes the detection of fluorescent signals. This spectral approach not only improves sensitivity but also simplifies the design and analysis of multicolor applications. Unlike traditional flow cytometers that rely on color compensation to subtract overlapping fluorescence, the SA3800 employs spectral unmixing. This method combines the fluorescence data from all channels and then mathematically separates the colors, yielding clearer and more accurate data. This process simplifies workflow, improves the visualization of autofluorescence, and enhances overall data quality.

Innovative Optical Technologies

The SA3800 features a unique prism-based collection system that directs emitted light to a 32-channel photomultiplier tube (PMT). This setup allows for the detection of 34 data points, capturing a comprehensive spectral fingerprint of each fluorochrome. The result is precise and accurate visualizations of fluorescent populations, providing researchers with detailed information about each marker’s spectral characteristics.

Spectral Unmixing

One of the standout features of the SA3800 is its ability to perform spectral unmixing. This powerful capability allows for the separation of overlapping fluorophores into pure signals, enabling more accurate measurement of each fluorochrome. Unlike conventional systems where overlapping signals can obscure data, spectral unmixing captures the full range of emitted photons, enhancing the detection of dim signals and rare populations. This technique also effectively handles autofluorescence by isolating and subtracting it from the signal, resulting in cleaner and more reliable data.

Automated Workflow

Automation is a key aspect of the SA3800, ensuring a streamlined and accurate workflow. The system is designed to handle a variety of standard and deep-well plates, as well as 12 x 75-mm 5-mL tubes. The novel 3D AutoSampler technology further enhances efficiency by moving the sample plate in both horizontal and vertical directions. This reduces sample-to-sample cross-contamination and speeds up cleaning processes. The fixed probe, combined with sensors that calculate the height of the sample container and automatically adjust for any contact, minimizes the risk of cross-contamination and flow problems, ensuring a reliable and consistent sample acquisition process.

User-Friendly Software

The SA3800 is equipped with intuitive software that simplifies every aspect of operation. From setup and panel design to data acquisition and analysis, the software provides step-by-step guidance. The user interface allows for customization of instrument settings and experiment configurations, accommodating both novice and experienced users. Key features include the Experimental Designer, which assists in creating experiments, and the Spectral Library, which facilitates the management of reagent data for future use.

System Standardization

The SA3800 includes a novel standardization feature that automatically sets the system to a master specification with a single click. This function eliminates variability between instruments, ensuring consistent and reliable results across different setups and locations. By reducing the potential for setup subjectivity, the standardization mode supports collaborative research and long-term studies, providing accurate and reproducible data regardless of operator experience.

Efficient Sample Acquisition and Analysis

The system’s software supports a range of acquisition functions, including the ability to control instrument settings and adjust acquisition speeds. The Walk Away Event Checker monitors the integrity of the sample stream, detecting issues such as air bubbles or low event rates, and can automatically clear clogs or continue to the next sample. The Autofluorescence Finder feature helps identify and subtract autofluorescent signals, ensuring that researchers obtain accurate representations of their fluorescent populations.

FCS Express Integration

The SA3800 comes with fully integrated FCS Express™ software from De Novo Software, providing advanced analysis tools for detailed data interpretation. This software supports a range of functions, including live gating, spectral overlays, tSNE, Spade, and heat maps. Live gating enables users to quickly and accurately identify positive and negative populations, enhancing the reliability of target population detection.

System Shutdown and Maintenance

At shutdown, the SA3800 software guides users through pre-cleaning procedures and automatically shuts down the instrument. The system also includes software wizards for bleach cleaning and rinsing, ensuring that the instrument is properly maintained and ready for future use.

In summary, the Sony SA3800 spectral cell analyzer stands out for its integration of advanced spectral technology, robust automation, and user-friendly software. Its ability to handle complex multicolor workflows with precision and ease makes it an ideal choice for research facilities seeking a high-performance, versatile analyzer. By combining cutting-edge optical technologies with comprehensive automation and intuitive software, the SA3800 offers a powerful solution for cellular acquisition and analysis, facilitating groundbreaking research in cellular biology.

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Unparalleled Spectral Technology for Advanced Sorting

At the heart of the FP7000 is Sony’s patented spectral technology, which sets it apart from conventional flow cytometry and cell sorting systems. Unlike traditional systems that rely on specific optical filters for each fluorochrome, the FP7000 employs spectral technology that captures the entire emission spectrum of each fluorochrome, enabling the detection of more than 44 colors simultaneously. This capability is further enhanced by the system’s ability to detect autofluorescence signals from 6 lasers—ranging from ultraviolet to near-infrared wavelengths. The FP7000 is equipped with 182 photomultiplier tube (PMT) detectors, ensuring comprehensive data acquisition and enabling researchers to analyze complex cell populations with high precision.

The system’s optical capabilities are closely aligned with Sony’s ID7000™ Spectral Cell Analyzer, allowing for smooth assay transfer between analysis and sorting. This feature is particularly beneficial for researchers who need to replicate their analyses on different instruments, ensuring consistency and reliability in their experiments. The FP7000’s optical system is also capable of detecting particles as small as 100 nanometers, making it suitable for a wide range of applications, from basic research to clinical diagnostics.

High-Performance Sorting for Diverse Applications

One of the standout features of the FP7000 is its high-speed, real-time spectral unmixing capability, which allows for the simultaneous detection and sorting of cell populations at rates of up to 25,000 events per second. This high throughput is achieved without compromising accuracy, thanks to the system’s patented unmixing algorithm. This algorithm is designed to resolve closely spaced fluorochromes with exceptional precision, eliminating the need for traditional compensation techniques, which can be subjective and prone to error. As a result, the FP7000 produces high-quality, reproducible data that is essential for complex multicolor experiments.

The FP7000 supports a wide range of nozzle sizes, which can be easily interchanged to accommodate different cell types and sorting requirements. This flexibility is crucial for researchers working with diverse samples, as it allows for optimized sorting conditions tailored to the specific needs of each experiment. The system’s sheath excitation technology ensures robust operation across different nozzle sizes, forming stable droplets at frequencies ranging from 12kHz to 100kHz and at pressures between 70 kPa and 600 kPa. This versatility enables the FP7000 to perform both bulk sorting into tubes and high-precision single-cell sorting into multiwell plates, making it suitable for a variety of downstream applications.

Automation and Workflow Integration

The FP7000 is designed with automation in mind, making it accessible to researchers of varying expertise levels. One of the key features of the system is the CoreFinder™ algorithm, which automates several critical steps in the sorting process. This includes optical alignment, laser delay optimization, droplet calibration, sort delay optimization, and side stream adjustment. By automating these processes, the FP7000 eliminates the subjectivity associated with manual adjustments, ensuring consistent performance and reducing the likelihood of operator error. This automation not only streamlines the workflow but also significantly reduces the time required for instrument setup, allowing researchers to focus on their experiments rather than on the technical aspects of the sorter.

In addition to CoreFinder™, the FP7000 features guided workflows that further simplify the operation of the system. These workflows include the Spectral Reference Library, which provides a comprehensive database of spectral signatures for commonly used fluorochromes. This library can be used to streamline the setup of experiments, making it easier for researchers to select the appropriate settings for their specific applications. The system also includes an autofluorescence unmixing feature, which enables the identification and removal of autofluorescence signals from the data. This is particularly important when working with heterogeneous samples that may contain cells or particles with high autofluorescence, as it improves the resolution of dim signals and enhances the overall quality of the data.

Real-Time Data Analysis and Flexibility

The FP7000 is not only a powerful sorter but also an advanced analytical tool. The system’s real-time spectral unmixing capability allows for the live analysis of data as it is being acquired, providing researchers with immediate feedback on their experiments. This real-time analysis is complemented by the system’s ability to perform 6-way sorting, which enables the isolation of multiple cell populations simultaneously. This feature is particularly useful for researchers who need to sort complex samples into multiple subsets for downstream applications, such as single-cell sequencing or functional assays.

The system’s flexibility extends to its data output formats, which are compatible with a wide range of third-party analysis software. This ensures that researchers can easily integrate the FP7000 into their existing workflows without the need for extensive reformatting or data conversion. The FP7000’s user-friendly interface and intuitive software design make it easy to learn and use, even for those with limited experience in spectral cell sorting. The software includes customizable wizards and guided protocols that assist users in setting up experiments, performing routine maintenance, and troubleshooting common issues.

Meeting the Needs of Modern Laboratories

The FP7000 Spectral Cell Sorter is designed to meet the diverse needs of modern laboratories, from basic research to complex, high-parameter applications. Its combination of advanced spectral technology, high-performance sorting, and automated workflows makes it an indispensable tool for researchers seeking to push the boundaries of cell sorting and analysis. Whether you are working with simple two-color assays or complex panels with more than 44 colors, the FP7000 provides the reliability, precision, and flexibility needed to achieve your research goals.

In conclusion, the FP7000 Spectral Cell Sorter represents a significant advancement in the field of flow cytometry and cell sorting. With its unparalleled spectral technology, robust performance, and user-friendly design, the FP7000 is well-equipped to handle the most demanding sorting applications, making it a valuable asset for any laboratory. Whether you are conducting basic research or pursuing cutting-edge applications, the FP7000 provides the tools and capabilities needed to deliver high-quality, reproducible results with confidence.

The ID7000™ spectral cell analyzer represents a significant advancement in high-parameter flow cytometry, offering unparalleled capabilities for comprehensive cell analysis. This state-of-the-art system excels in detecting both dim and rare cell populations with high sensitivity, making it a valuable tool for researchers aiming to explore complex biological questions. Equipped with up to seven lasers and 186 detectors—the most of any flow cytometer currently available—the ID7000 supports experiments with 44 colors or more, depending on the fluorochromes used. This extensive configuration allows for the detailed examination of heterogeneous cell populations and accommodates future advancements in fluorochrome technology. The laser options range from deep ultraviolet (320 nm) to infrared (808 nm), ensuring versatility and the capability to expand multicolor panels. The system’s advanced detection features facilitate precise and accurate data collection, streamlining high-parameter experiments and multicolor workflows. Innovative elements such as the Spectral Reference Library and the Autofluorescence Finder enhance data fidelity by identifying and subtracting autofluorescence contributions, thus ensuring more accurate and reliable results.

Designed for Multicolor Workflows

The ID7000’s advanced optics design, alongside proprietary technologies, delivers exceptional data quality and resolution. Sony has leveraged its expertise in spectral technology to develop signal processing methods that minimize electronic noise, which is crucial for achieving high sensitivity in flow cytometry. This high sensitivity enables the accurate detection of rare and dim signals, thus facilitating deeper insights into cellular biology. The new excitation and detection optics are tailored to resolve spectrally similar fluorochromes with precision. The system features three standard excitation lasers—488 nm (blue), 637 nm (red), and 405 nm (violet)—with optional lasers at 561 nm (yellow-green), 355 nm (ultraviolet), 320 nm (deep ultraviolet), and 808 nm (infrared). Each laser is optimized to maximize performance for detecting various markers, including those with low signal intensity.

The expanded availability of detectors is designed to handle the complexity of panel design and fluorochrome selection, empowering researchers to push the boundaries of discovery. The ID7000 employs photomultiplier tubes (PMTs) known for their high signal-to-noise ratios to capture photons effectively. Emitted light is collected across a wide spectrum (360 nm to 920 nm) using an innovative light diffraction grating-based collection system. This system enhances photon capture and provides high resolution, ensuring that even the faintest signals are detected. By distributing light evenly across the detector array, the system minimizes light loss and maximizes data accuracy.

Designed to Harness the Power of Spectral Data

The ID7000 spectral cell analyzer simplifies complex operations and enhances the accuracy of spectral analysis. Spectral unmixing, a key feature of the ID7000, separates overlapping signals into pure fluorochrome signals, enabling precise measurement of each marker. The system uses the Weighted Least Squares Method (WLSM) to perform unmixing, which is essential for analyzing dim and rare markers. This technology simplifies the design of multicolor panels by eliminating the need for traditional bandpass filters and compensation matrixes, reducing complexity and potential errors in data interpretation.

Additionally, the ID7000 addresses the challenge of autofluorescence, which can interfere with the signals from fluorescent markers. Spectral technology enables autofluorescence to be treated as a separate color, allowing for accurate subtraction and clearer visualization of true fluorescent populations. The system’s Spectral Reference Library stores single-color control data, which is used to enhance the unmixing process. This library eliminates the need for repetitive controls in each experiment, saving time and resources.

The ID7000 also features a standardization mode to ensure reproducible data across experiments and instruments. This mode optimizes instrument settings to maintain consistency, supporting longitudinal studies and cross-site collaborations. By adjusting PMT voltages and other parameters, the system minimizes variability and enhances data reliability.

Designed for Automated Sample Acquisition

Automation is a core feature of the ID7000, designed to streamline operations and ensure accurate results. The advanced AutoSampler accommodates various plate and tube formats, including 5-mL tube racks and 96-well plates, and allows for sample cooling and agitation. The AutoSampler’s flexibility includes options for different wash protocols and sample handling modes, ensuring that samples remain stable and accurate throughout the acquisition process. Additional features include an extra loading station for QC particles or cleaning solutions, enhancing convenience and efficiency during multi-sample acquisitions.

The ID7000’s software is user-friendly, guiding researchers through setup, acquisition, and analysis with ease. It offers expert and guided modes to accommodate users with varying levels of experience. The software supports customizable workflows and automated data acquisition, reducing the need for manual intervention and allowing researchers to focus on their scientific work.

Automated Setup and Quality Control

The ID7000 includes automated setup and quality control features to ensure optimal performance. At startup, the system uses wizards for alignments and performance checks, providing real-time feedback and generating reports for tracking instrument performance. The History and Trend views in the QC tab allow users to monitor performance over time, ensuring consistency and reliability in data collection. These features are crucial for maintaining high standards in research and supporting reproducible results across different experiments and laboratories.

Overall, the ID7000 spectral cell analyzer combines cutting-edge spectral technology with robust automation and user-friendly software, making it an ideal choice for researchers seeking to advance their work in high-parameter flow cytometry. Its ability to handle complex multicolor workflows, combined with powerful data analysis tools and automation, positions it as a leading solution for exploring and understanding cellular biology in unprecedented detail.